slidehyb ii hybridization buffer (Thermo Fisher)
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Slidehyb Ii Hybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slidehyb/us11642396-191-12-16
Average 86 stars, based on 1 article reviews
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Labeling:Article Title: High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis Article Snippet: The amino allyl modified cDNA was synthesized with the Superscript III Reverse Transcriptase kit (Invitrogen), purified with Cyscribe GFX purification kit (Amersham Biosciences), labeled with Cy3 and Cy5 dyes and purified again. .. The labeled cDNA was hybridized to oligonucleotide microarrays in Article Title: Carbohydrate Availability Regulates Virulence Gene Expression in Streptococcus suis Article Snippet: RNA (1 μg) from S. suis samples was labeled using the Cyanine 5 (Cy5) labeling reaction (Amersham Biosciences, Buckinghamshire, UK). .. Co-hybridization with labeled cDNA probes was performed on these oligonucleotide arrays at 42°C for 16 h in Article Title: The Songbird Neurogenomics (SoNG) Initiative: Community-based tools and strategies for study of brain gene function and evolution Article Snippet: Template DNA was polymerized from 4 μg digested and cleaned DNA using high concentration Klenow (New England Biolabs) and incorporating amino-allyl dUTP (Sigma Aldrich) for 4 hours at 37°C. .. The resulting amino-allyl labeled DNA was coupled with Cy dye (GE Healthcare), cleaned, and hybridized using Hybridization:Article Title: The Songbird Neurogenomics (SoNG) Initiative: Community-based tools and strategies for study of brain gene function and evolution Article Snippet: Template DNA was polymerized from 4 μg digested and cleaned DNA using high concentration Klenow (New England Biolabs) and incorporating amino-allyl dUTP (Sigma Aldrich) for 4 hours at 37°C. .. The resulting amino-allyl labeled DNA was coupled with Cy dye (GE Healthcare), cleaned, and hybridized using |
